Per BBC Sport, he joins fellow tennis players Milos Raonic and Simona Halep in withdrawing from the Games.
The 24-year-old Pliskova told the Czech News Agency she was pulling out for the same reason as Berdych.
Berdych, a Wimbledon semifinalist, said, "It was a hard decision for me to take after a long and painstaking discussion" with family.
On Twitter, the Czech says "to limit health risks towards my dearest is the utmost priority".
The 24-year-old Romanian wrote on her Facebook page, "I regret to announce that I have decided I will not compete in the Olympic Games". I hope the Romanian Olympic and Sports Committee and all Romanians will respect my decision. I played in malaria countries where malaria kills way more people than Zika even thinks about.
"John Isner of the United States, Austria's Dominic Thiem, Australian duo Bernard Tomic and Nick Kyrgios as well as Spanish veteran Feliciano Lopez have all opted, for various reasons, not to take part in the Olympics".
Raonic - beaten by Andy Murray in his first grand slam final - is the first top-10 male tennis player to rule himself out of Rio, as the sport makes its eighth Olympic appearance since returning to the schedule in 1988.
A number of the top women's players have expressed their excitement for the event, however, including world number one Lydia Ko.
The Czech is the third tennis player to announce their withdrawal from the tournament due to the unsafe virus.
The ace was one of two eagles on the day for Vegas, who will compete for Venezuela at the Rio Olympics next month, to go with eight birdies and just one bogey.
Serena Williams, who earned her 22nd Grand Slam title at Wimbledon, will defend the singles and doubles titles she captured in London.
The Chinese delegation was unveiled here on Monday as 160 men's athletes and 256 women will compete in 210 events over 26 sports at the Games which open on August 5 in Rio de Janeiro.
Croatian Dario Saric has signed with the Philadelphia 76ers, the team said, two years after acquiring the rights to the highly-rated forward.
Meanwhile. The International Association of Athletics Federations' (IAAF) actions suggest the organisation had got orders from "somebody" not to allow any Russians at any cost at the Rio Olympics, two-time Olympic pole vault champion Yelena Isinbaeva said on Sunday.