There were no injuries or damage reported but the Israeli fire was seen as a warning to Syria to pay careful attention that mortar fire not hit the Golan Heights, which Israel acquired from Syria in the 1967 war and later annexed.
On August 22, the air force hit what it said was a Syrian army rocket launcher in response to "stray fire from Syrian conflict". "The IDF will continue to act in order to safeguard Israel and its civilians".
Israeli war planes have bombed Syrian army positions in Syria's Golan Heights for the second time in less than a week.
Since the signing of a 1974 disengagement agreement between Syria and Israel, a cease-fire line has separated the Israeli-occupied Golan Heights from a Syria-administered buffer zone. Syria wants the return of the territory that United Nations peacekeeping forces have monitored for decades.
The Syrian Golan has been the site of intense fighting in recent years between Assad regime forces and the al-Nusra Front, an al-Qaeda affiliate.
Syria says Israel and its Western and regional allies are aiding militant groups operating inside the Arab country. A 2014 IDF statement said its forces "have experienced combat in the region, both from targeted strikes and errant fire".
The IDF said in a statement that it held Syrian authorities responsible for all actions that take place within its borders, adding that the IDF "will not allow any attempt to affect Israel's sovereignty or the safety of its people".
Last year, for example, Israeli forces struck a auto in a Syrian-controlled area of the Golan Heights, killing five civilians, Syrian state media reported.