They also have key striker Jonas back and firing after he missed the group stage with a knee injury. However, Andrija Zivkovic is suspended.
Tuchel shuffled his pack for the Darmstadt match, naming three teenagers in his starting lineup and leaving Andre Schürrle, Ousmane Dembele and Gonazalo Castro on the bench.
Borussia Dortmund and Barcelona travel to Benfica and PSG respectively on Tuesday night, before Arsenal take on Bayern Munich, while Real Madrid host Napoli on Wednesday.
Tuesday's fixture at the Estadio da Luz will be the first meeting between Benfica and Dortmund since the first round of the 1963/64 European Cup, a tie the German club won 6-2 on aggregate.
"In the last 16 of the Champions League there is never a favourite", he said.
Dortmund are already struggling to make the cut for next year's Champions League after winning only 9 of their 20 matches in this season.
Dortmund will try to overcome the 1-0 deficit in the return leg on March 8.
However, Dortmund have lost six of their last eight games in the Champions League knockout stages (W2).
"Hardly any player will play 500 games for Benfica again".
"Aubameyang is not fully fit after the African Nations Cup and his body language did not suggest that he would do better after giving away (missing) the penalty", Dortmund coach Thomas Tuchel said of the forward. He's a raw athletic talent and he'll enjoy the Champions League because it should be more open and he's getting quite closely marked in the Bundesliga.
Raph Honigstein joins the FC show to breakdown Dortmund's loss to Benfica, and Aubameyang's poor performance. The visitors are 12/1 sixth favourites in the outright, while their hosts are the joint longest priced side of those left the competition at 100/1.
Aubameyang wasted a handful of chances to get the Bundesliga side into the game in open play and when his terrible penalty was easily saved, Tuchel responded by withdrawing his top-scorer from the pitch nearly half-an-hour early.
"It would be great to win the match, and for him to score".
Here's what he's predicting in his Sky Bet tips for the four clashes.