It also includes tombs and a funerary building, reports Daily Mail.
Kholi said this was an unprecedented discovery as it was "the first human necropolis found in central Egypt with so many mummies".
They also found a golden sheet and two papyri in Demotic, an ancient Egyptian script, as well as a number of sarcophogi made of limestone and clay.
The burial chamber was first detected a year ago by a team of Cairo University students using radar.
The minister of antiquities, Khaled al-Enany, announced the discovery on the site in the city of Minya, around 250 kilometres south of Cairo.
The artefacts date back to the Greco-Roman era stretching from 332 BC to 395 AD that started with Alexander the Great's conquest of Egypt.
Egypt's tourism sector, which relies heavily on its ancient history, has been badly hit by political turmoil since the 2011 uprising. The announcement took place at a press conference Saturday morning in Tuna el-Gebel district in Al-Minya.
Mummies lying in catacombs following their discovery in the Tuna el-Gabal district.
Yehia Rashed, Egypt's tourism minister said last month that the new finds could boost tourist arrivals this year to about 10 million, an improvement from the 9.3 million visitors that came in 2015 but still far below the 14.7 million from 2010. No 2016 figure is yet available.
El-Enany told reporters about this week's cachette discovery at a gala ceremony attended by El-Minya governor Essam Al Bedewi, the ambassadors of Belgium, Hungary and Serbia and a number of top officials from the ministry and Cairo University.