Le Pen, who won a seat in the National Assembly for the first time in four attempts, was aiming to reach the 15-seat threshold, a feat which would grant her party greater financial support and more speaking time.
The winning margin is lower than some expected, with turnout down from 2012. Macron's party, which didn't exist 14 months ago and offered novice candidates from civilian life, has drawn from left and right to fill its ranks, effectively blurring the traditional left-right political divide.
Macron's twin victories in last month's presidential election and in Sunday's parliamentary vote marks the routing of the old political class.
The conservative party had enough seats to "defend its convictions", said the party's leader for the elections, Francois Baroin, calling on Macron to heed the record low turnout, which he said sent "a message".
Correspondents say opponents of Mr Macron may simply have not bothered to turn out.
Polling agency projections suggested Macron's Republic on the Move!
What do the results show?
REM and its centrist ally MoDem won 350 seats in the 577-seat assembly.
Les Republicains ("The Republicans"), the mainstream conservative party in France, also sank to an all-time low of 137 seats, losing 92, according to early election results.
He has already had little pushback on his stated intention to use executive orders to push through reforms without parliamentary debate though street protests over the erosion of cherished workers' rights such as those seen a year ago are considered likely.
Even where women do run for seats, they tend to be in constituencies where they are unlikely to win, keeping the numbers of women who make it to the Palais-Bourbon low.
Socialist leader Jean-Christophe Cambadelis admitted a "historic defeat" for his party and resigned from his post.
"He will also face an enormous amount of resistance on the ground from the vested interests of the trade unions which still wield an enormous amount of influence, and could make life very hard for the inexperienced new President and his party", said Michael Hewson, chief market analyst at CMC Markets UK.
People are looking at their new leader, and many more than voted for him are honestly impressed by his calibre. Also, social mobility is higher in France than in the U.S., for example.
There is an unknown aspect to the coming mandate that sets it apart from all that went before.
Le Pen's victory in the northern former coalmining town of Henin-Beaumont was a rare bright spot for her nationalist and anti-EU party that was once hoping to emerge as the principal opposition to Macron.
What happened in some of the key races?
Macron, who was elected at age 39 as the youngest president in France's history, has now fulfilled his wish to disrupt politics with a new approach and new faces, - including a farmer, a teacher, and a math genius.