A recent report from The Telegraph says that associates of Gatlin were approached by undercover reporters about obtaining illegal performance-enhancing drugs.
Gatlin - who was banned for doping in 2001 and again in 2006 - was booed by spectators in August this year after his controversial win over favourite Usain Bolt in the World Athletics Championship.
Listen to the latest news on our 5 radio stations Legend FM, Viti FM, Radio Sargam, FM96 and Navtarang. The reporter said he was making a film about running and wanted to give its star some PEDs to improve his physical attributes. Wagner also allegedly said that Gatlin had been using performance-enhancing drugs.
"We are presently coordinating with the Athletics Integrity Unit in order to investigate these claims fully".
The coach, the former Olympic gold medallist Dennis Mitchell, and the agent, Robert Wagner, were also secretly recorded claiming that the use of banned substances in athletics was still widespread as they described how positive doping tests could be avoided.
Justin Gatlin was booed by spectators after defeating Usain Bolt at the 2017 World Athletics Championships in London
"Under the IAAF Rules, all athlete support personnel - agents, coaches etc - are bound by both the IAAF anti-doping code and IAAF integrity code of conduct". Wagner reportedly claimed that all track and field athletes doped, although he later said that he was just playing along with the supposed producer to "get the job".
Gatlin's legal representatives said the sprinter had sacked Mitchell and said he had more than five years' worth of official drugs tests to show "he has never tested positive for any banned substance", the paper reported.
Head of the Athletics Integrity Unit, Brett Clothier said there would be an investigation into the claims, in co-operation with USADA. "As with all investigations, we encourage individuals with information to come forward as an important tool to help protect clean athletes".
Wagner also denied the charges, saying: "I wasn't involved in doping". Importantly, individuals are innocent unless and until the established process determines otherwise.
IAAF president Lord Coe described the allegations as "serious", adding: "I know the independent Athletics Integrity Unit will investigate in accordance with its mandate".