The sheriff's office said the deputy, who has not been identified by the office, was responding to a neighbor dispute on Baltimore Way and made contact with a resident.
According to details given by Highland County Sheriff Paul Blackman during a press conference, Deputy William Gentry responded to reports of a cat being shot near a residence in Lake Placid on Sunday around 7:45 p.m.
A Highlands County deputy remains in critical condition at Lee Memorial hospital where he was airlifted after being shot in the line of duty Sunday evening.
Blackman said Ables shot Gentry in the head just before 8pm.
Officials say the deputy was critically injured.
Deputy Gentry, 40, has nine years and four months of service with the Highlands County Sheriff's Office.
The Sheriff's Office said that Ables has been charged with: "Attempted first degree murder without premeditation; Possession of a weapon by a convicted felon; Resisting an officer with violence; Tampering with evidence; Violation of probation". Deputies said Ables is a convicted felon with a history of violence towards law enforcement.
Florida Gov. Rick Scott said Monday that he and his wife, Ann, "join all Floridians in mourning the loss of another Florida hero, Deputy William Gentry, whose life has now been senselessly taken". The sheriff says Gentry's brother is also a deputy at the Highlands County Sheriff's Office.
Lake Placid is about two hours southeast of Tampa.
"Please keep his family and our HCSO family in your prayers", the sheriff's office tweeted in announcing his death.