The Republican judge, who lost his bid for Senate a year ago after being accused of initiating sexual relationships with teenagers while in his 30s, filed the lawsuit Wednesday in the D.C. District Court. He has denied the allegations.
Larry Klayman, a conservative lawyer representing Moore, in a statement called his client "a man of great faith, morality and intellect", and said the defendants will be held legally accountable for Baron Cohen's conduct.
They are seeking $95m (£73m) in damages from Baron Cohen and from the Showtime and CBS networks.
Now, he's filing a lawsuit against Sacha Baron Cohen, CBS and Showtime after claiming he was tricked into appearing on the show.
A NY judge in 2008 tossed out lawsuits brought by a driving instructor and two etiquette school teachers who said they were duped into appearing in the movie "Borat" in which Baron Cohen plays an awkward foreign journalist traveling the United States.
As part of his complaint, Moore claims he would not have agreed to fly to D.C.to take part in the episode (which, obviously), and that he has suffered defamation, intentional infliction of emotional distress, and fraud, due to the prank.
"The press has been sent copies of an alleged complaint, yet to our knowledge SHOWTIME has not been served".
According to the lawsuit, Cohen, "while in character, falsely and fraudulently induces unsuspecting victims, such as Judge Moore to be interviewed under dishonest, unethical, illegal and false pretenses".
"I've been married for 33 years and never had an accusation of such things", Moore said. "Tonight, is the last EVER show of Who is America?".
In another episode, former Arizona sheriff and Senate candidate Joe Arpaio argued the benefits of gun ownership to a tiny toy doughnut.
Roy Moore in a still from 'Who Is America?'
Baron Cohen then said they also created a device which could detect "pedophiles" after merely picking up on their scent. The judge said they accepted money and signed agreements releasing the filmmakers from liability. The suit alleges that the release was obtained on fraudulent grounds. The interview has already seen the light of TVs and, as of this writing, is still up on YouTube.