According to Team 12 political insider Brahm Resnik, Gosar's siblings broke ties with him after controversial comments he made following the deadly white nationalist rally in Charlottesville in the summer of 2017.
"I think my brother has traded a lot of the values we had at our kitchen table", she said later.
Mr Gosar, who is one of ten children, has not yet responded to this most recent public attack by his siblings.
"It's intervention time. And intervention time means that you go to vote, and you go to vote Paul out", says Tim Gosar, a private investigator.
In one ad, which percolated on Twitter on Friday after being unveiled at a Democratic fundraiser the day before, the siblings do not immediately reveal their identities.
Gosar, 59, R-Ariz., is running for reelection in Arizona's 4th Congressional District.
In this December 2013, file photo, US Representative Paul Gosar, R-Ariz., speaks during a Congressional Field Hearing on the Affordable Care Act in Apache Junction, Ariz.
"Where is his integrity?" asked sis Jennifer Gosar in an ad she filmed for her brother's Democratic opponent, David Brill. It is remarkable, however, when those disagreements are aired in a campaign ad. "Stalin would be proud".
"We've got to stand up for our good name", David Gosar said in another ad.
He added, "To the six angry Democrat Gosars - see you at Mom and Dad's house!"
Gosar is one of President Donald Trump's biggest defenders, but his siblings do not appear to share his political views.
The Phoenix New Times reported the campaign contacted Gosar's siblings for the ad after a staffer saw David Gosar's tweets criticizing his brother. Gosar also told Vice that 88-year-old Soros, who survived German-occupied Hungary, had "turned in his own people to the Nazis"-a common slander against Soros by conspiracy theorists".
Brill acknowledged that speaking against their brother was not easy for the Gosar siblings. The Congressman also believes that the white supremacist rally held at Charlottesville past year was a creation of the left. He told the Phoenix New Times he is grateful for the Gosar family's support, calling them "upstanding, wonderful people". His brother Pete, a Democrat who did not participate in the Brill campaign videos, ran unsuccessfully for Wyoming governor in 2014.
The website FiveThirtyEight lists Gosar's district as strongly leaning Republican, and gives the Congressman a greater than 99.9 percent chance of being re-elected.