The Juventus forward also missed Portugal's first two post-World Cup matches last month, with coach Fernando Santos saying Ronaldo had only just moved to the Italian club and was still settling into life in Turin.
After missing the European tie in midweek, following his red card in Valencia last month, Ronaldo should return to the team this weekend as Juve look to maintain their 100 per cent domestic record.
The Italy worldwide is delighted to be a teammate of the Portugal captain at Juventus, and insisted he has always preferred Ronaldo over Messi.
Meanwhile Portuguese Football Federation president Fernando Gomes has given his "total solidarity" towards Ronaldo as "his good name and reputation are being questioned".
On Thursday, Portugal coach Fernando Santos said Ronaldo also will not be called up for the next round of global games in November.
Neither Ronaldo nor Juventus have addressed the allegation by Kathryn Mayorga that she received a payoff of $375,000 in 2010 after being put under pressure by the footballer's "fixers" to keep quiet about an incident in a penthouse hotel suite.
Sportswear maker Nike Inc (NKE.N) and video game maker Electronic Arts Inc (EA.O) and said on Thursday they were concerned about rape allegations against Cristiano Ronaldo, one of the world's best football players.
"When Cristiano Ronaldo completed the sexual assault of the plaintiff, he allowed her to leave the bedroom stating he was sorry, he was usually a gentleman", the lawsuit said.
Ronaldo, through his Twitter, faulted the media for riding on the explosive allegations, adding that his conscience was clear. It is learnt that she decided not to stay out in the public because of her emotional state and trauma she had undergone.
"In the future, nothing will prevent Ronaldo from contributing to the team", Santos said at a news conference.
The five-time Ballon d´Or victor missed the two internationals Portugal played in September.