There is a strong charity element to Rooney's inclusion as it is hoped it will help raise around £500,000 for the Wayne Rooney Foundation, which helps various charities including the NSPCC.
The friendly game against the United States on 15 November will give Rooney the chance to win his 120th cap and will also raise funds for the Wayne Rooney Foundation.
Having made his worldwide debut in 2003 as England's youngest ever player, Rooney surpassed Bobby Charlton as his country's leading scorer in 2015.
He said at the time: "It was great that Gareth Southgate called me this week to tell me he wanted me back in the England squad for the upcoming matches". Playing for England has always been special to me. "It's fitting that the match will be against the US and I hope that both sets of fans enjoy the game".
The game against the USA - three days before the decisive Nations League clash with Croatia at Wembley - has been designated as a charity fund-raiser by the FA. Trying to help others is a particular focus of mine now through my Foundation.
The former England worldwide also became his country's top scorer and now plays in the MLS with D.C United following a short spell at Everton. Their season ended on Thursday night when they suffered a penalty shootout defeat to Columbus Crew in the first round of the play-offs.
"I am truly humbled and hugely excited to play for England at Wembley again, ” said Rooney". "Acknowledging the Three Lions legacy has been a major part of our journey with this current group of young players and Wayne Rooney is a hugely significant part of that history".