The end was nigh when Konta blazed a forehand drive volley over the baseline in another moment reminiscent of the French Open to drop serve again in the sixth game, and a final error sealed her fate.
Williams ended the brilliant grass-court run of fellow American Alison Riske with a hard-earned 6-4 4-6 6-3 victory over Ashleigh Barty's fourth-round slayer in a rollercoaster quarter-final. "So I am not really scared to play her".
Thirty-three-year-old Strycova, who is ranked 54, said earlier this week it may be her final Wimbledon, and she now moves through to a first grand slam singles semi-final to take on Serena Williams.
In the bottom half the former world No 1 Simona Halep of Romania takes on the Ukrainian Elina Svitolina, two of the best athletes and competitors in the game.
Serena Williams is in a good place, pain-free at last and 100 per cent focused on winning Wimbledon, her coach Patrick Mouratoglou said Wednesday.
"I'm no less of a person or a player if I don't get past this point".
The Spaniard said: "She hits very fast, so I can not be calm and I make more unforced errors than I usually do". Svitolina might normally be favored on grass, but Halep says she's feeling more stable-with her feet and her mind-on the slippery surface than she has in the past. "I just will go there Thursday and I will try to play my game", Strycova said.
Serena Williams and Barbora Strycova meet in the semifinal of the 2019 tennis Wimbledon.
But Williams is tennis tungsten, and recovered superbly from a brief wobble and a taped ankle in which she lost the second set and fell a break behind in the third.
The Romanian, last year's French Open champion, last made the Wimbledon final four in 2014 when she was beaten by Eugenie Bouchard of Canada.
Konta doesn't like going into big matches as the favourite, and she doesn't like opponents who mess her about with changes of rhythm, pace and spin.
I've enjoyed Wimbledon every time I've had the opportunity to play - it is just a little bit more stressful when you're playing singles. But I will take it like I took every match since I'm here. I couldn't quite find the level that I needed to make it hard and challenging for the kind of player she is.
The Czech player didn't look back as Konta had no response to her brilliant tactical approach. Melichar, meanwhile, captured last year's Wimbledon mixed doubles title along with Alexander Peya, beating Murray and Victoria Azarenka in the final.